Analysis of timepoint data

The Customer

• June 03, 2019 05:58

Hello,

Hopefully somebody can help us. We have imaged cells (2 fields of view per well) over a timecourse using the CV8000 (see screenshot). We want to observe differences in cell area over time after addition of a compound. When we analyse this data we do not get a value for each timepoint (16 timepoints = 16 values), we only get 1 value per well. I am not sure whether we are analysing only 1 timepoint or if all the timepoints averaged, it is difficult to work out. I think we are not completing the "Define Results" building block correctly. If anyone has any guidance for this it would be greatly appreciated.

Kind regards,

Caroline

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  The Customer

  • June 04, 2019 10:23

  This is really helpful, thank you!

  I can see all the timepoint data when I export the batch analysis results.

  Many thanks,

  Caroline

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  Connor Wood

  • June 03, 2022 07:26

  We are having this same issue, it looks like it was resolved, but I do not see the solution that worked. If there was something missing from the define results building block of the analysis, we would be grateful to know what it was. 

   

   

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  Richard Howell

  • June 15, 2022 15:02

  If executing the analysis script via the Image Analysis view, Columbus will only measure those fields/timepoints/planes which have been explicitly selected, which by default would be only the single field of view that is displayed on screen at the time. If you want the output for multiple timepoints you'd need to tell Columbus, by right-clicking on the "Timepoint" section of the data selection pane and selecting Multiple or All timepoints. The results output will then be generated for the selected timepoints, and would be available via the Columbus UI. 

  If executing the script using the Batch Analysis functionality (which would be the preferred method if analysing more that a few fields at a time), then ALL images are measured by default, theres no need to make any specific selections. In order to access the timepoint data however you'd need to export the results files via the Export view, or publish the data and then download the result files of interest.

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